1ZZN
Crystal structure of a group I intron/two exon complex that includes all catalytic metal ion ligands.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 108.466, 108.466, 246.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.040 - 3.370 |
R-factor | 0.269 |
Rwork | 0.269 |
R-free | 0.30700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1u6b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.490 |
High resolution limit [Å] | 3.370 | 3.370 |
Rmerge | 0.072 | |
Number of reflections | 20658 | |
<I/σ(I)> | 18.2 | 1.1 |
Completeness [%] | 95.9 | 71.1 |
Redundancy | 5.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | MPD, sodium cacodylate, magnesium acetate, potassium chloride, cobalt heximine, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |