1ZZC
Crystal Structure of CoII HppE in Complex with Tris Buffer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 86.360, 86.360, 220.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.390 - 1.800 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.24200 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 45155 | |
<I/σ(I)> | 35 | 3.9 |
Completeness [%] | 98.2 | 99.9 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | pH 8.5 |