1ZYP
Synchrotron reduced form of the N-terminal domain of Salmonella typhimurium AhpF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.943, 107.943, 85.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.270 - 2.400 |
| Rwork | 0.205 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 19094 | |
| Completeness [%] | 94.3 | 86.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 30-35% PEG 4000, 0.2M ammonium acetate, 0.1M Tris , pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
