1ZYK
Anthranilate Phosphoribosyltransferase in complex with PRPP, anthranilate and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 92.556, 66.099, 117.069 |
| Unit cell angles | 90.00, 107.76, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.207 |
| R-free | 0.26900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o17 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 52279 | |
| <I/σ(I)> | 15.33 | 5.45 |
| Completeness [%] | 96.9 | 87.1 |
| Redundancy | 3.76 | 3.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 1500, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
