1ZXO
X-ray Crystal Structure of Protein Q8A1P1 from Bacteroides thetaiotaomicron. Northeast Structural Genomics Consortium Target BtR25.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97908,0.97932,0.96797 |
| Spacegroup name | H 3 |
| Unit cell lengths | 158.154, 158.154, 275.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.560 - 3.200 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.29700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.083 | 0.549 |
| <I/σ(I)> | 24.64 | 3.2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | 8% PEG8K, 0.1M NaHepes, 8% Ethyl-Glycol , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
