1ZXN
Human DNA topoisomerase IIa ATPase/ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2004-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.015, 90.492, 148.293 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.510 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Number of reflections | 61096 | |
| Completeness [%] | 100.0 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | PEG 1500, ammonium sulfate, tris chloride, ADP, magnesium chloride, DTT, pH 8.0, VAPOR DIFFUSION, temperature 293K |
