1ZXN
Human DNA topoisomerase IIa ATPase/ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 160 |
Detector technology | CCD |
Collection date | 2004-08-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.015, 90.492, 148.293 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.510 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.510 | 2.510 |
Number of reflections | 61096 | |
Completeness [%] | 100.0 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | PEG 1500, ammonium sulfate, tris chloride, ADP, magnesium chloride, DTT, pH 8.0, VAPOR DIFFUSION, temperature 293K |