1ZXK
Crystal Structure of Cadherin8 EC1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 46.196, 39.934, 66.439 |
| Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.25358 |
| Rwork | 0.252 |
| R-free | 0.28336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zvn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.439 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.078 | 0.201 |
| Number of reflections | 15229 | |
| <I/σ(I)> | 10.4 | 3.9 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 22% PEG 4000, 0.1M HEPES pH7, 0.1M NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
