1ZWY
Crystal structure of protein VC0702 from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97970 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.399, 105.347, 75.272 |
Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.18272 |
Rwork | 0.180 |
R-free | 0.22552 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.481 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.066 | 0.684 |
Number of reflections | 63342 | |
<I/σ(I)> | 21.15 | 1.84 |
Completeness [%] | 98.4 | 95.2 |
Redundancy | 6.2 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG 3000, HEPES, NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 395K |