1ZW5

X-ray structure of Farnesyl diphosphate synthase protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-10
Detector	MARRESEARCH
Wavelength(s)	0.984
Spacegroup name	P 41 21 2
Unit cell lengths	112.255, 112.255, 63.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.17508
Rwork	0.172
R-free	0.22311
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yv5
RMSD bond length	0.015
RMSD bond angle	1.504
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.100
Number of reflections	17517
<I/σ(I)>	13.9	2.7
Completeness [%]	95.6	97.9
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	14% PEG 6000, 0.7 M LiCl, 70 mM citrate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K