1ZVU
Structure of the full-length E. coli ParC subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1157 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 257.990, 62.141, 63.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.24323 |
Rwork | 0.240 |
R-free | 0.29583 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1AB4 (E. coli GyrA NTD) related entry 1ZVT (E. coli ParC CTD) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.087 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 19320 | |
<I/σ(I)> | 9.5 | 2.5 |
Completeness [%] | 89.7 | 89.1 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, NaCl, glycerol, PEG-6000, 1,3-butanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |