1ZVP
Crystal Structure of a Protein of Unknown Function VC0802 from Vibrio cholerae, Possible Transport Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-21 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.224, 99.561, 163.433 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.650 - 2.200 |
| R-factor | 0.21573 |
| Rwork | 0.214 |
| R-free | 0.24643 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.327 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.720 |
| Number of reflections | 40583 | |
| <I/σ(I)> | 17.44 | 1.28 |
| Completeness [%] | 95.1 | 72.2 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 45% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
