1ZVM

Crystal structure of human CD38: cyclic-ADP-ribosyl synthetase/NAD+ glycohydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 2 2 21
Unit cell lengths	114.439, 115.704, 98.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.228
Rwork	0.228
R-free	0.27000
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	65067
<I/σ(I)>	12.6	2.6
Completeness [%]	96.9	90.1
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	291	PEG4000, Lithium sulfate, hexanediol, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.50