1ZVI

Rat Neuronal Nitric Oxide Synthase Oxygenase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	C 2 2 21
Unit cell lengths	43.183, 107.522, 163.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.202
Rwork	0.199
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	24173
<I/σ(I)>	11	4.4
Completeness [%]	92.3	79.5
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, DTT, PEG3350, EPPS, NACL, GLYCEROL, H4B, beta-MERCAPTOETHANOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K