1ZV5

Crystal structure of the variable domain of the camelid heavy-chain antibody D2-L29 in complex with hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	CCD
Collection date	2003-10-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.8123
Spacegroup name	P 43 21 2
Unit cell lengths	114.073, 114.073, 35.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.670 - 2.000
R-factor	0.217
Rwork	0.217
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ENTITY 1: CHAIN L OF 1JTT ENTITY 2: 1YCV
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.056	0.425
Number of reflections	16498
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	Ammonium sulphate, PEG 400, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K