1ZV2
Cu-containing nitrite reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.007 |
| Spacegroup name | H 3 |
| Unit cell lengths | 72.381, 72.381, 147.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.700 - 1.740 |
| R-factor | 0.15667 |
| Rwork | 0.155 |
| R-free | 0.19367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nif |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.438 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.700 | 1.810 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.065 | 0.340 |
| Number of reflections | 29034 | |
| <I/σ(I)> | 9.7 | 2.9 |
| Completeness [%] | 98.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | PEG4000, MgCl2, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
