1ZUZ
Calmodulin in complex with a mutant peptide from human DRP-1 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.813 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.520, 34.030, 73.850 |
| Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.910 |
| R-factor | 0.1941 |
| Rwork | 0.191 |
| R-free | 0.24829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wrz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.115 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 12230 | |
| <I/σ(I)> | 19.9 | 3.8 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | PEG3350, MPD, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
