1ZUK
Yeast BBC1 Sh3 domain complexed with a peptide from Las17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.813 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.770, 78.500, 35.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.17645 |
Rwork | 0.174 |
R-free | 0.22599 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tg0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.279 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.950 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 12659 | |
<I/σ(I)> | 15.3 | 2.8 |
Completeness [%] | 98.4 | 98.5 |
Redundancy | 4.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | VAPOR DIFFUSION, HANGING DROP, temperature 295K |