1ZU5
Crystal structure of FtsY from Mycoplasma mycoides- space group H32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91 |
Spacegroup name | H 3 2 |
Unit cell lengths | 148.441, 148.441, 223.925 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.21156 |
Rwork | 0.210 |
R-free | 0.24642 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB CODE 1ZU4 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.674 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 35370 | |
Completeness [%] | 95.0 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | Sodium citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |