1ZTU
Structure of the chromophore binding domain of bacterial phytochrome
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2004-10-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793, 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.876, 133.666, 49.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.23887 |
| Rwork | 0.237 |
| R-free | 0.26569 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.817 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.056 | 0.111 |
| Number of reflections | 15209 | |
| <I/σ(I)> | 14.2 | 10.8 |
| Completeness [%] | 97.0 | 90.4 |
| Redundancy | 6.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.95 | 298 | PEG 400, sodium acetate, pH 4.95, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 4.95 | 298 | PEG 400, sodium acetate, pH 4.95, VAPOR DIFFUSION, temperature 298K |
