1ZTP
X-ray structure of gene product from homo sapiens Hs.433573
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.547, 116.809, 123.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.19118 |
| Rwork | 0.188 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.422 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.106 | 0.597 |
| Number of reflections | 31664 | |
| <I/σ(I)> | 13.353 | 2.675 |
| Completeness [%] | 98.6 | 93.1 |
| Redundancy | 12.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10 MG/ML PROTEIN, 1.2 M SODIUM CITRATE, 0.100 M TRIS, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
