1ZTH
Crystal Structure of A.fulgidus Rio1 serine protein kinase bound to ADP and Manganese ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.414, 80.084, 121.056 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.890 |
| R-factor | 0.19224 |
| Rwork | 0.189 |
| R-free | 0.26215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ztf |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.602 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.939 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.350 | |
| Number of reflections | 78499 | |
| Completeness [%] | 79.58 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 4000, MES, Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 293K |
