1ZT2
Heterodimeric structure of the core primase.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.28242 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 193.287, 193.287, 213.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.120 - 3.330 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.845 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.120 | 3.470 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Number of reflections | 59500 | |
| <I/σ(I)> | 17.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Tris hydrochloride, Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
