1ZSZ
Crystal structure of a computationally designed SspB heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.004, 61.036, 62.385 |
| Unit cell angles | 90.00, 110.87, 90.00 |
Refinement procedure
| Resolution | 29.150 - 2.000 |
| R-factor | 0.242 |
| Rwork | 0.231 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ou9 chain A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.371 |
| Number of reflections | 28612 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | PEG 6000, CaCl2, sodium cacodylate, KCl, glycerol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
