1ZSQ

Crystal Structure of MTMR2 in complex with phosphatidylinositol 3-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1.009
Spacegroup name	P 41 21 2
Unit cell lengths	66.227, 66.227, 261.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.820
Rwork	0.218
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lw3
RMSD bond length	0.013
RMSD bond angle	2.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.820	1.820
Number of reflections	52572
<I/σ(I)>	21.9	3.5
Completeness [%]	98.0	85
Redundancy	13	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 35000, Tris, TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K