1ZSH
Crystal structure of bovine arrestin-2 in complex with inositol hexakisphosphate (IP6)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 78.880, 78.880, 158.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.800 - 2.900 |
Rwork | 0.250 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jsy |
RMSD bond length | 0.013 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.800 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 14737 | |
<I/σ(I)> | 17.8 | |
Completeness [%] | 99.6 | 97.7 |
Redundancy | 16.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Magnesium Formate, HEPES, Polypropylene glycol 400, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |