1ZS9

Crystal structure of human enolase-phosphatase E1

Replaces:  1WDH

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-09
Detector	MARRESEARCH
Wavelength(s)	0.9000, 0.9798, 0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	54.017, 57.546, 87.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.208
Rwork	0.208
R-free	0.24100
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.520
Data reduction software	AUTOMAR
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.770
High resolution limit [Å]	1.700	1.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	PEG4000, Hepes, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K