1ZS3
The crystal structure of the Lactococcus lactis MG1363 DpsB protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2001-05-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 127.977, 128.379, 193.903 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.700 |
R-factor | 0.20941 |
Rwork | 0.207 |
R-free | 0.25779 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qgh |
RMSD bond length | 0.022 |
RMSD bond angle | 1.692 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CCP4 |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.074 | 0.407 |
Number of reflections | 85912 | |
<I/σ(I)> | 7.5 | 1.7 |
Completeness [%] | 97.8 | 97.4 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.0M Sodium Formate, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.0M Sodium Formate, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |