1ZS2
Amylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.983 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.741, 116.358, 61.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.220 - 2.160 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g5a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.095 | 0.238 |
| Number of reflections | 37336 | |
| <I/σ(I)> | 13 | 4.3 |
| Completeness [%] | 99.2 | 96.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 6000, SODIUM CHLORIDE, TRIS-HCL, EDTA, HEPES, DTT, sucrose, maltoheptaose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
