1ZRT

Rhodobacter capsulatus cytochrome bc1 complex with stigmatellin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-03-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.0800
Spacegroup name	P 1 21 1
Unit cell lengths	95.633, 154.360, 103.057
Unit cell angles	90.00, 113.57, 90.00

Refinement procedure

Resolution	59.770 - 3.510
R-factor	0.224
Rwork	0.221
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bcc
RMSD bond length	0.019
RMSD bond angle	2.187
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (dev_3885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.120	0.280
Number of reflections	33596	3197
<I/σ(I)>	8.5	3.9
Completeness [%]	97.6	94
Redundancy	2.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	277	PEG-400, 0.1 M MgCl2, Heptanetriol, Cacodylate, Undecylmaltoside
1	VAPOR DIFFUSION, SITTING DROP	5.2	277	PEG-400, 0.1 M MgCl2, Heptanetriol, Cacodylate, Undecylmaltoside
1	VAPOR DIFFUSION, SITTING DROP	5.2	277	PEG-400, 0.1 M MgCl2, Heptanetriol, Cacodylate, Undecylmaltoside