1ZR5
Crystal structure of the macro-domain of human core histone variant macroH2A1.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.93100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.549, 72.258, 144.641 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.360 - 2.920 |
| R-factor | 0.2165 |
| Rwork | 0.213 |
| R-free | 0.27691 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model of macroH2A1.2 based on PDB-ID 1HJZ |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.360 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 9713 | |
| <I/σ(I)> | 19.7 | 4.46 |
| Completeness [%] | 97.5 | 84.9 |
| Redundancy | 6.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG 5000MME 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
