1ZR5

Crystal structure of the macro-domain of human core histone variant macroH2A1.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-18
Detector	MARRESEARCH
Wavelength(s)	0.93100
Spacegroup name	P 21 21 21
Unit cell lengths	40.549, 72.258, 144.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.360 - 2.920
R-factor	0.2165
Rwork	0.213
R-free	0.27691
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model of macroH2A1.2 based on PDB-ID 1HJZ
RMSD bond length	0.015
RMSD bond angle	1.640
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.360	3.080
High resolution limit [Å]	2.900	2.900
Number of reflections	9713
<I/σ(I)>	19.7	4.46
Completeness [%]	97.5	84.9
Redundancy	6.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	25% PEG 5000MME 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K