1ZR4
Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently linked to two Cleaved DNAs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.990 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.158, 127.290, 140.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.26554 |
| Rwork | 0.264 |
| R-free | 0.29469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.550 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.047 | 0.010 |
| Number of reflections | 33091 | |
| <I/σ(I)> | 25.7 | 2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 303 | 0.2M NaCl, 0.1M Magnesium Formate, 0.1M Sodium Acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Chloride | ||
| 2 | 1 | 1 | Magnesium Formate | ||
| 3 | 1 | 1 | Sodium Acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium Chloride | ||
| 6 | 1 | 2 | Magnesium Formate | ||
| 7 | 1 | 2 | Sodium Acetate | ||
| 8 | 1 | 2 | H2O |
