1ZQ1
Structure of GatDE tRNA-Dependent Amidotransferase from Pyrococcus abyssi
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97576,0.97956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.700, 138.200, 134.400 |
| Unit cell angles | 90.00, 109.60, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.2171 |
| Rwork | 0.217 |
| R-free | 0.25600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.069 | 0.374 |
| Number of reflections | 137974 | |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 297 | PEG 20000, Dioxane, Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
