1ZPW
Crystal structure of a hypothetical protein TT1823 from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-05 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 44.234, 55.964, 74.937 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.460 - 1.640 |
R-factor | 0.18595 |
Rwork | 0.185 |
R-free | 0.21259 |
Structure solution method | MR, MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.587 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 11711 | |
Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 22% PEG 3350, 0.1M TrisCl pH 7.6, 0.2M NaF, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 22% PEG 3350, 0.1M TrisCl pH 7.6, 0.2M NaF, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 22% PEG 3350, 0.1M TrisCl pH 7.6, 0.2M NaF, VAPOR DIFFUSION, HANGING DROP, temperature 293K |