1ZPU

Crystal Structure of Fet3p, a Multicopper Oxidase that Functions in Iron Import

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-20
Detector	CUSTOM-MADE
Wavelength(s)	1.37837
Spacegroup name	P 32
Unit cell lengths	168.534, 168.534, 174.605
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.22721
Rwork	0.226
R-free	0.25742
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.411
Data reduction software	HKL-2000
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	136782
<I/σ(I)>	14.9	2.3
Completeness [%]	99.6	99.9
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium citrate, HEPES, ethylene glycol, ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium citrate, HEPES, ethylene glycol, ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	sodium citrate, HEPES, ethylene glycol, ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K