1ZPD

PYRUVATE DECARBOXYLASE FROM ZYMOMONAS MOBILIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	69.868, 92.168, 98.256
Unit cell angles	102.86, 94.59, 112.69

Refinement procedure

Resolution	15.000 - 1.860
R-factor	0.162 *
Rwork	0.162
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1YPD
RMSD bond length	0.007
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.800	1.910
High resolution limit [Å]	1.860	1.860
Rmerge	0.063	0.210
Total number of observations	306481 *
Number of reflections	157263	13826 *
<I/σ(I)>	11.1	2.6
Completeness [%]	88.2 *	51.7 *
Redundancy	1.91	1.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	20 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	dithiothreitol	1 (mM)
3	1	reservoir	Mes-NaOH	100 (mM)
4	1	reservoir	PEG1500	24 (%(w/v))