1ZP4
Glu28Gln mutant of E. coli Methylenetetrahydrofolate Reductase (oxidized) complex with Methyltetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97623 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.590, 127.810, 97.950 |
| Unit cell angles | 90.00, 121.88, 90.00 |
Refinement procedure
| Resolution | 17.270 - 1.850 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b5t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.270 | 1.910 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 91462 | |
| <I/σ(I)> | 14.41 | 3.8 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.6 | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG 4000, LITHIUM SULFATE, SODIUM CACODYLATE, ETHANOL, MESO-ERYTHRITOL, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
