1ZOR

Isocitrate dehydrogenase from the hyperthermophile Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-28
Detector	MARRESEARCH
Wavelength(s)	0.996
Spacegroup name	P 21 21 21
Unit cell lengths	62.470, 88.051, 180.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.560 - 2.240
R-factor	0.18647
Rwork	0.184
R-free	0.22317
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lwd 1t0l
RMSD bond length	0.012
RMSD bond angle	1.251
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.560	2.290
High resolution limit [Å]	2.240	2.240
Rmerge	0.066	0.379
Number of reflections	48990
<I/σ(I)>	15.84	3.32
Completeness [%]	99.7	100
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	PEG 6000, Sodium Acetate, Succinate, NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K