1ZOL
native beta-PGM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.760, 57.190, 75.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.910 - 1.900 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lvh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.910 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.137 |
| Number of reflections | 18379 | |
| <I/σ(I)> | 15.4 | 5.4 |
| Completeness [%] | 98.6 | 96.7 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100 mM Tris HCl pH 8.5, 200 mM sodium acetate, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
