1ZNC
HUMAN CARBONIC ANHYDRASE IV
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.500, 47.700, 141.000 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | POLYALANINE MODEL OF CAII MISSING N-TERMINAL 30 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.253 |
| Total number of observations | 46250 * | |
| Number of reflections | 13626 | |
| Completeness [%] | 95.0 | 91.8 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.1 | 25% PEG 3,350; 100MM NA ACETATE PH 5.1 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | Tris sulfate | 50 (mM) | |
| 3 | 1 | reservoir | PEG3350 | 25 (%) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | benzamidine | 2 (mM) | |
| 6 | 1 | reservoir | n-octyl beta-D-glucopyranoside | (mM) |
