1ZMO
Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.980, 77.720, 111.422 |
| Unit cell angles | 97.29, 89.97, 112.98 |
Refinement procedure
| Resolution | 20.050 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pwx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 120343 | |
| Completeness [%] | 89.4 | 89.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% w/v PEG 8000, 100 mM MES buffer, 100 mM Magnesium Acetate, 50 mM Sodium Formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
