1ZM5
Conjugative Relaxase TRWC in complex with ORIT dna, cooper-bound structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97855 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 91.077, 91.077, 205.551 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.680 - 2.500 |
R-factor | 0.261 |
Rwork | 0.233 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omh |
RMSD bond length | 0.005 |
RMSD bond angle | 1.520 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.680 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.104 |
Number of reflections | 18252 |
Redundancy | 21.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG MME 2000, Sodium acetate, Ammonium sulfate, Cooper (II) chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG MME 2000 | ||
2 | 1 | 1 | Sodium acetate | ||
3 | 1 | 1 | Ammonium sulfate | ||
4 | 1 | 1 | Cooper (II) chloride | ||
5 | 1 | 2 | Sodium acetate | ||
6 | 1 | 2 | PEG MME 2000 |