1ZLH
Crystal structure of the tick carboxypeptidase inhibitor in complex with bovine carboxypeptidase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-07 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.070, 68.600, 57.890 |
| Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
| Resolution | 55.900 - 1.700 |
| R-factor | 0.15561 |
| Rwork | 0.154 |
| R-free | 0.18532 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.120 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.060 | 0.134 |
| Number of reflections | 34176 | |
| Completeness [%] | 87.0 | 61.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M sodium cacodylate, 0.2M zinc acetate dihydrate, 7%(w/v) PEG 8000, 10%(w/v) dried dioxan, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
