1ZKZ

Crystal Structure of BMP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	I 41 2 2
Unit cell lengths	71.234, 71.234, 144.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.800 - 2.330
R-factor	0.23254
Rwork	0.231
R-free	0.27181
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bmp
RMSD bond length	0.017
RMSD bond angle	1.866
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.800	50.000	2.400
High resolution limit [Å]	2.320	5.000	2.320
Rmerge	0.077	0.045	0.357
Number of reflections	8142
Redundancy	13.5	12.6	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	1-1.2 M Sodium Chloride, 7-10 mM Hexadecyltrimethylammonium Bromide 10 mM Magnesium Chloride, pH 7.5, temperature 296K, VAPOR DIFFUSION, HANGING DROP