1ZKY
Human Estrogen Receptor Alpha Ligand-Binding Domain In Complex With OBCP-3M and A Glucocorticoid Receptor Interacting Protein 1 Nr Box II Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-04-07 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.929, 84.129, 58.220 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 27.010 - 2.250 |
| R-factor | 0.18756 |
| Rwork | 0.185 |
| R-free | 0.23141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l2i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.048 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.118 | 0.564 |
| Number of reflections | 24141 | |
| <I/σ(I)> | 10.759 | 2 |
| Completeness [%] | 59.7 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M sodium malonate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
