1ZKQ

Crystal structure of mouse thioredoxin reductase type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	I 41 2 2
Unit cell lengths	108.830, 108.830, 205.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.400 - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6v
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	d*TREK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.086	0.440
Number of reflections	19370
<I/σ(I)>	18.7	6.5
Completeness [%]	100.0	100
Redundancy	15.88	15.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	100 MM MES, 24% PEG 550 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K