1ZK8
Crystal structure of transcriptional regulator from Bacillus cereus ATCC 14579
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.268, 95.640, 45.986 |
| Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.150 |
| R-factor | 0.18466 |
| Rwork | 0.182 |
| R-free | 0.23858 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 18311 | |
| Completeness [%] | 94.0 | 92.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 15 % Peg 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
