1ZJR

Crystal Structure of A. aeolicus TrmH/SpoU tRNA modifying enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1271
Spacegroup name	P 61 2 2
Unit cell lengths	66.658, 66.658, 194.884
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.237
Rwork	0.216
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gz0
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.058	0.473
Number of reflections	21822
<I/σ(I)>	47.3	2.9
Completeness [%]	95.3	65
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	308	sodium citrate, ammonium sulfate, PEG 100, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 308K