1ZJL
Crystal structure of zinc-bound engineered maltose binding protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2005-03-06 |
| Detector | UCSD MARK III |
| Wavelength(s) | 1.282995 |
| Spacegroup name | P 32 |
| Unit cell lengths | 80.500, 80.500, 55.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.860 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.148 |
| Number of reflections | 27349 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000 MME, sodium acetate, zinc acetate, HEPES, sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
