1ZJJ
Crystal structure of hypothetical protein PH1952 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2004-12-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.717, 75.743, 79.784 |
| Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.850 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wvi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.057 | 0.217 |
| Number of reflections | 40186 | |
| <I/σ(I)> | 11.5 | 3.1 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.8 | 291 | PEG 4000, HEPES, magnesium chloride, pH 6.8, MICROBATCH, temperature 291K |
