1ZJ7
Crystal structure of a complex of mutant HIV-1 protease (A71V, V82T, I84V) with a hydroxyethylamine peptidomimetic inhibitor BOC-PHE-PSI[S-CH(OH)CH2NH]-PHE-GLU-PHE-NH2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9340 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 62.859, 62.859, 83.253 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.930 |
R-factor | 0.2287 |
Rwork | 0.229 |
R-free | 0.29940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iiq |
RMSD bond length | 0.012 |
RMSD bond angle | 1.707 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.048 | 0.434 |
Number of reflections | 6784 | |
<I/σ(I)> | 28.1 | 4.4 |
Completeness [%] | 87.6 | 85.4 |
Redundancy | 6.7 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 0.1 M Na Citrate, 0.5 M NaCl, 10% (v/v) glycerol, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |